期刊
CANCER LETTERS
卷 169, 期 1, 页码 51-58出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/S0304-3835(01)00558-4
关键词
cAMP-dependent protein kinase; nucleotide excision repair; protein kinase; regulation; cisplatin; drug resistance; phosphorylation; cAMP
类别
Enhanced DNA repair is an important factor in drug resistance in cancer. Using cell-free extracts derived from the fission yeast, Schizosaccharomyces pombe, we demonstrate in an in vitro system DNA repair system that increased cAMP levels, which activates cAMP-dependent protein kinase (PKA), inhibits repair of ultraviolet (UV)-damaged DNA. Supplementing the cell-free system with the catalytic kinase subunit of PKA also inhibits DNA repair. In contrast, addition of the PKA inhibitor H-89 enhances repair activity. These results show that PKA regulates DNA repair synthesis, thus implicating the cAMP signaling pathway in DNA damage response and repair of UV-damaged DNA lesions. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
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