High-performance affinity capture-removal of bacterial pyrogen from solutions

Synthetic peptide S3 Delta has high affinity for bacterial endotoxin or lipopolysaccharide (LPS). Under tested conditions of pH 5-9 and 0-0.4 M NaCl, the affinity constant, K-D ranged from 2.10(-6) to 2.10(-9) M-1. A novel affinity matrix based on peptide S3 Delta was developed for removal of LPS from solutions such as: water; buffers with a wide range of ionic strength and pH; medium for cell culture: and protein solutions under optimized conditions. At a starting LPS of approximate to 100 EU/ml, a post-purification level below 0.005 EU/ml was achieved. (C) 2001 Elsevier Science B.V. All rights reserved.