期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 46, 期 3, 页码 217-225出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(01)00276-7
关键词
Vibrio bacteriophage; generalized transduction; Vibrio cholerae; CP-T1ts
资金
- NIAID NIH HHS [AI40262] Funding Source: Medline
- NIDDK NIH HHS [P30 DK34928] Funding Source: Medline
CP-T1 is the only described generalized transducing bacteriophage for the intestinal pathogen Vibrio cholerae. yet many of its basic biological parameters remain unknown. Due to low frequencies of transduction and pseudolysogen formation, CP-T1 has not been widely used as a genetic tool. To overcome these limitations, we have isolated a conditional mutant of CP-T1 that exhibits temperature-sensitive plaque formation. Several biological properties of CP-T1ts were determined, including its restrictive temperature, adsorbance profile to host cells, burst time, and burst sire. Based on these properties, an optimized transduction protocol was designed which resulted in several fold higher transduction frequencies for a variety of genetic markers from a number of chromosomal loci. Generalized transduction was also demonstrated between classical and El Tor biotype strains of V. cholerae. (C) 2001 Elsevier Science B.V. All rights reserved.
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