期刊
JOURNAL OF BASIC MICROBIOLOGY
卷 55, 期 10, 页码 1232-1238出版社
WILEY
DOI: 10.1002/jobm.201500008
关键词
Heavy metals; ACCD; Herbaspirillium sp; RT-qPCR
类别
资金
- National Research Foundation of Korea (NRF) - government (MEST) [2011-0020202]
- Korea Ministry of Environment as Eco innovation project [2013001470006]
This study aimed to understand the influence of heavy metals on 1-aminocyclopropane-1-carboxylate deaminase activity (ACCD) and acdS gene expression in Herbaspirillium sp. GW103. The GW103 strain ACCD activity decreased in cells grown in a medium supplemented with Pb and As, whereas cells grown in medium supplemented with Cu showed increase in enzyme activity. The GW103 strain produced 262.2 +/- 6.17 Rmol of a-ketobutyrate per milligram of protein per hour during ACC deamination at 25 degrees C after 24 h incubation. Using a PCR approach, an acdS codinggene of 1.06 kbp was amplified in isolate GW103, showing 92% identity with Herbaspirillum seropedicae SmR1 acdS gene. Real time quantitative polymerase chain reaction results indicate that the acdS expression rate was increased (7.1-fold) in the presence of Cu, whereas it decreased (0.2- and 0.1-fold) in the presence of As and Pb.
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