期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 52, 期 362, 页码 1779-1784出版社
OXFORD UNIV PRESS
DOI: 10.1093/jexbot/52.362.1779
关键词
antisense; photosynthesis; Rubisco; SBPase
The response of net photosynthetic CO2 uptake (A) to increasing leaf intercellular CO2 concentration (ci) was determined in antisense Nicotiana tabacum plants, derived from six independent transformation lines, displaying a range of sedoheptulose-1, 7-bisphosphatase (SBPase) activities. The maximum in vivo ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) carboxylation (V-c,V-max) and RuBP regeneration (J(max)) rates were calculated from the steady-state measurements of the A to ci response curves. In plants with reductions in SBPase activity of between 9% and 60%, maximum RuBP regeneration capacity declined linearly (r(2) = 0.79) and no significant.,m J change in apparent in vivo Rubisco activity (V-c,V-max) was observed in these plants. No correlation between V-c,V-max and a decrease in capacity for RuBP regeneration was observed (r(2) = 0.14) in the SBPase antisense plants. These data demonstrate that small decreases in SBPase activity limit photosynthetic carbon assimilation by reducing the capacity for RuBP regeneration.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据