期刊
BIOINFORMATICS
卷 17, 期 9, 页码 838-839出版社
OXFORD UNIV PRESS
DOI: 10.1093/bioinformatics/17.9.838
关键词
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Primer-introduced restriction analysis (PIRA-PCR) is widely used to detect Single Nucleotide Polymorphisms (SNPs). To create artificial Restriction Fragment Length Polymorphism (RFLP), a mismatch is usually introduced near the 3' end of the primer that is close to the mutation of interest. We describe in this report a www-based computer program that screens for the suitable mismatches, designs the primers, lists the appropriate restriction enzymes and other related information.
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