期刊
JOURNAL OF VIROLOGY
卷 75, 期 18, 页码 8854-8858出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.75.18.8854-8858.2001
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资金
- NIAID NIH HHS [AI18449, R56 AI018449, AI36884, R21 AI018449, AI22795, R01 AI022795, R01 AI018449] Funding Source: Medline
Varicella-zoster virus (VZV) codes for a protein serine kinase called ORF47; the herpes simplex virus (HSV) homolog is UL13. No recombinant alphaherpesvirus serine kinase has been biologically active in vitro. We discovered that preservation of the intrinsic kinase activity of recombinant VZV ORF47 required unusually stringent in vitro conditions, including physiological concentrations of polyamines. In this assay, ORF47 phosphorylated two VZV regulatory proteins: the ORF62 protein (homolog of HSV ICP4) and the ORF63 protein (homolog of HSV ICP22). Of interest, ORF47 kinase also coprecipitated ORF63 protein from the kinase assay supernatant.
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