期刊
JOURNAL OF BASIC MICROBIOLOGY
卷 56, 期 1, 页码 59-66出版社
WILEY-BLACKWELL
DOI: 10.1002/jobm.201500417
关键词
Streptomyces phytase; Phosphate limitation; PhoR; PhoP; PHO box; Negative; Positive regulation; Beta-glucuronidase reporter gene
类别
资金
- Tunisian Government (Contract Program LMB-CBS)
- CMCU Chouayekh/Virolle [11G0924]
- Energetic Metabolism of Streptomyces
Sco7697, a gene encoding a phytase, enzyme able to degrade phytate (myo-inositol 1,2,3,4,5,6-hexakis phosphate), the most abundant phosphorus storing compound in plants is present in the genome of S. coelicolor, a soil born bacteria with a saprophytic lifestyle. The expression of this gene was previously shown to be induced in conditions of Pi limitation by the response regulator PhoP binding to an operator sequence, the PHO box, located upstream of the -35 promoter sequence. A close examination of the promoter region of sco7697 revealed the presence of another putative operator site, a Direct Repeat (DR), located downstream of the - 10 promoter sequence. In order to determine whether this DR played a role in regulation of sco7697 expression, different variants of the phytase gene promoter region were transcriptionally fused to the beta-glucuronidase reporter gene (GUS). As expected, deletion of the PHO box led to abolition of sco7697 induction in conditions of Pi limitation. Interestingly, alteration of the DR correlated with a dramatic increase of GUS expression but only when PhoP was present. These results demonstrated that this DR is the site of strong negative regulation by an unknown repressor. The latter would impede the necessary activation of phytase expression by PhoP.
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