期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 49, 期 9, 页码 1155-1164出版社
HISTOCHEMICAL SOC INC
DOI: 10.1177/002215540104900909
关键词
human placental syncytiotrophoblasts; placental alkaline phosphatase; BeWo cells
类别
Human placental alkaline phosphatase (PLAP) was localized at the apical and basal plasma membrane of syncytiotrophoblasts and at the surface of cytotrophoblasts in term chorionic villi using immunoelectron microscopy. Similarly, apical and basolateral PLAP expression was found in polarized trophoblast-derived BeWo cells. Trophoblasts isolated from term placentas exhibited mainly vesicular PLAP immunofluorescence staining immediately after isolation. After in vitro differentiation into syncytia, PLAP plasma membrane expression was upregulated and exceeded that observed in mononuclear trophoblasts. These data call for caution in using PLAP as a morphological marker to differentiate syncytiotrophoblasts from cytotrophoblasts or as a marker enzyme for placental brush-border membranes.
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