期刊
PHYTOCHEMISTRY
卷 58, 期 1, 页码 43-46出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(01)00191-1
关键词
Petroselinum crispum; Apiaceae; flavonoid biosynthesis; flavone synthase I cloning; 2-oxoglutarate-dependent dioxygenase; heterologous expression
A cDNA encoding flavone synthase I was amplified by RT-PCR from leaflets of Petroselinum crispum cv. Italian Giant seedlings and functionally expressed in yeast cells. The identity of the recombinant, 2-oxoglutarate-dependent enzyme was verified in assays converting (2S)-naringenin to apigenin. (C) 2001 Elsevier Science Ltd. All rights reserved.
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