期刊
PLANT MOLECULAR BIOLOGY REPORTER
卷 19, 期 3, 页码 227-233出版社
INT SOC PLANT MOLECULAR BIOLOGY
DOI: 10.1007/BF02772894
关键词
96-well format; DNA isolation; high throughput; paint shaker; RNA isolation
Most steps in plant nucleic acid isolation are easily adapted to 96-well format; however, tissue disruption typically is performed on samples individually and often is the rate-limiting step in sample processing. We have found that DNA and RNA isolation from Arabidopsis tissue can be carried out in 96-well format using a paint shaker fitted with an adapter for tissue disruption.
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