期刊
THEORETICAL AND APPLIED GENETICS
卷 103, 期 4, 页码 526-532出版社
SPRINGER-VERLAG
DOI: 10.1007/s001220100598
关键词
brown planthopper (BPH); BPH resistance gene; bph2; AFLP and STS markers; linkage map; rice (Oryza sativa L.)
The brown planthopper (BPH), Nilaparvata lugens Stal, is a significant insect pest of rice (Oryza sativa L.). bph2 is one of the 12 major BPH resistance aeries so far identified in several indica cultivars and two wild relatives. We have constructed a high-resolution linkage map as a foundation for map-based cloning of the bph2 locus. An advanced mapping population derived from a cross of 'Tsukushibare' (a susceptible japonica cultivar) with 'Norin-PL4' (an authentic bph2-introgression line) was used. Segregation analysis by the mass seedling test showed that bph2 behaved as a single dominant gene. Through bulked segregant analysis and linkage analysis, bph2 was located within a 3.2-cM region containing eight AFLP markers. One marker (KAM4) showed complete co-segregation with bph2, and bph2 was mapped within a 1.0-cM region delimited by KAM3 and KAM5, two flanking markers. KAM4 was converted into a PCR-based sequence-tagged-site (STS) marker and its co-segregation with bph2 was validated.
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