期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 45, 期 9, 页码 2598-2603出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.45.9.2598-2603.2001
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Psendomonas aeruginosa GW-1 was isolated in 2000 in South Africa from blood cultures of a 38-year-old female who developed nosocomial pneumonia. This isolate harbored a self-transferable ca. 100-kb plasmid that conferred an expanded-spectrum cephalosporin resistance profile associated with an intermediate susceptibility to imipenem. A beta -lactamase gene, bla(GES-2), was cloned from whole-cell DNA of P. acruginosa GW-1 and expressed in Escherichia coli. GES-2, with a pl value of 5.8, hydrolyzed expanded-spectrum cephalosporins, and its substrate profile was extended to include imipenem compared to that of GES-1, identified previously in Klebsiella pneumoniae. GES-2 activity was less inhibited by clavulanic acid, tazobactam and imipenem than GES-1. The GES-2 amino acid sequence differs from that of GES-1 by a glycine-to-asparagine substitution in position 170 located in the omega loop of Ambler class A enzymes. This amino acid change may explain the extension of the substrate profile of the plasmid-encoded beta -lactamase GES-2.
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