期刊
MOLECULAR ECOLOGY NOTES
卷 1, 期 3, 页码 188-190出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1471-8278.2001.00071.x
关键词
allelic ladder; genetic diversity; sequence-tagged microsatellite site (STMS); simple sequence repeat (SSR)
Using an enrichment procedure, we have cloned microsatellite repeats from black poplar (Populus nigra L.) and developed primers for microsatellite marker analysis. Ten primer pairs, mostly for trinucleotide repeats, produced polymorphic fragments in A nigra. Some of them also showed amplification in other poplar species. (P. deltoides, P. tricocarpa, P. tremula, P. tremuloides, P. candicans, P. lasiocarpa). The best six loci were tested on 23 P. nigra genotypes collected across Europe. The microsatellites produced up to 12 alleles per locus in this set, with observed heterozygosity between 0.32 and 0.91.
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