期刊
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
卷 60, 期 9, 页码 885-892出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jnen/60.9.885
关键词
hippocampus; HIV; HIV-associated dementia; HIV encephalitis; laser capture microdissection; polymerase chain reaction (PCR)
资金
- NICHD NIH HHS [N01-HD83284] Funding Source: Medline
- NINDS NIH HHS [R01 NS35331, R01 NS39177] Funding Source: Medline
We employed laser capture microdissection to remove individual pyramidal neurons from the CA1, CA3, and CA4 regions of formalin-fixed, paraffin-embedded hippocampus from 8 AIDS brains and 2 HIV-1-seronegative normal brains. We amplified HIV-1 gag and nef gene sequences using separate, double round PCR reactions for each of the primer sets. In all 3 bippocampal regions, amplification efficiency was best with sequence length between 284 and 324 bp; HIV-1 nef gene sequences were more common than HIV-1 gag sequences; and rank order for percent positive amplification was CA3>CA4 >CA1 samples. These results are the first to detect HIV-1 gene sequences in microdissected human tissue. They indicate that brain neurons in vivo contain HIV-1 DNA sequences consistent with latent infection by this virus, and suggest that neurons display a selective vulnerability for HIV infection. Neuronal HIV infection could contribute to neuronal injury and death or act as a potential viral reservoir if reactivated.
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