期刊
IMMUNOLOGY AND CELL BIOLOGY
卷 79, 期 5, 页码 444-453出版社
WILEY
DOI: 10.1046/j.1440-1711.2001.01031.x
关键词
adherence; in situ hybridization; interleukin 1 beta; messenger RNA; monocyte
Differences in IL-1 beta mRNA expression, stability and translation between non-adherent monocytes and those stimulated by adherence suggest that cytokine regulation is coupled to the function and assembly of cytoskeletal structures. In situ hybridization studies were performed to visualize expression and positioning of IL-1 beta mRNA in adherently cultivated monocytes. IL-1 beta mRNA expression was heterogeneous with high transcript levels found in spread or polarized cells. Transcripts were compartmentalized to the perinuclear region in spread cells, and partially redistributed with polarization. In contrast to mRNA distribution in other motile cell populations, IL-1 beta mRNA did not localize to the distal or proximal actin cytoskeleton. Perinuclear confinement of transcripts required intact actin microfilaments. Treatment with cytoskeleton disruption and detergent extraction suggested that most non-translated IL-1 beta mRNA was associated with intermediate filaments. In monocytes stimulated by LPS, IL-1 beta, but not IL-1Ra transcripts were redistributed and partially associated, yet not bound to actin microfilaments. The present study demonstrates that IL-1 beta mRNA expression and localization in adherent monocytes is interrelated with the cytoskeletal rearrangement upon adherence, spreading and polarization.
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