期刊
PLANT AND CELL PHYSIOLOGY
卷 42, 期 9, 页码 969-975出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pce125
关键词
betacyanin; DOPA; Portulaca grandiflora; polyphenol oxidase (EC 1.10.3.1); pterin; tyrosine hydroxylase (EC 1.14.16.2)
Tyrosine hydroxylase was separated from polyphenol oxidase activity and was highly purified from betacyanin producing callus cultures of Portulaca grandiflora. The purified enzyme catalyzed the formation of DOPA (L-3,4-dihydroxyphenylalanine) from tyrosine and required the pterin compounds (6-methyl-5,6,7,8-tetrahydropterin; 5,6,7,8-tetrahydrobiopterin; 6,7-dimethyl-5,6,7,8-tetrahydropterin) as coenzyme. The K-m values for tyrosine and 6-methyl-5,6,7,8-tetrahydropterin were 0.5 mM and 0.15 mM, respectively. This enzyme was activated by Fe2+ and Mn2+, and inhibited by metal chelating agents.
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