期刊
APPLIED SURFACE SCIENCE
卷 181, 期 1-2, 页码 149-159出版社
ELSEVIER
DOI: 10.1016/S0169-4332(01)00384-1
关键词
carboxylate surface group; fluorescein probe; hot imprinting; microchannel; poly(methyl methacrylate); UV laser ablation
The physical morphology and chemical functionality of fluid microchannels formed in poly(methyl methacrylate) (PMMA) substrates were studied to increase the fundamental understanding of polymer microchannel surface properties for 'lab-on-a-chip' devices. Microchannels were formed by a hot-imprint method using a silicon template or by a laser ablation process (248 nm KrF laser) operating at low to moderate fluence levels (up to 1180 mJ/cm(2)). The carboxylate groups, which are responsible for the surface charges, were fluorescently labeled by reaction with an ethyl-dimethylaminopropyl-carbodiimide hydrochloride/amino-fluorescein solution. Fluorescence microscopy was then used to locate and measure qualitatively the charge present on the microchannel walls. Results suggest that surface charges are localized on the corners of trapezoidal channels formed by the hot-imprint method and that the amount of charge present is significantly less compared to laser-ablated microchannels where charges appear to be distributed uniformly. For substrates irradiated at fluences above the laser ablation threshold, it was found that one pass of the laser produced a surface with greater charge than channels made with multiple passes, that ablation under nitrogen resulted in more charge than ablation under oxygen, and that non-sonicated substrates had more charge than samples that were sonicated after ablation. Trends in the data for sonicated samples are explained through scanning electron microscopy images showing etch depth and UV-laser penetration to depths below the surface of the formed microchannel. Finally, we have determined that the surface charge on the substrate can be modified by using the laser at fluence levels lower than those required to ablate the substrate. (C) 2001 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据