期刊
NEUROSCIENCE LETTERS
卷 310, 期 1, 页码 61-65出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3940(01)02082-1
关键词
electrophysiology; cerebellum; Purkinje cell; metabotropic glutamate receptors; excitatory postsynaptic potential; LY367385; MPEP
Purkinje neurons were recorded from rat cerebellar slices. Parallel fibres stimulation elicited a fast excitatory postsynaptic potential (EPSP) mediated by ionotropic glutamate (iGluR) alpha -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors followed by the inhibitory gamma-aminobutyric acid(A) (GABA(A))-dependent postsynaptic potential. In the presence of antagonists for iGluRs and for GABA(A) receptors, brief tetanic activation evoked a slow metabotropic glutamate receptor (mGluR)-dependent EPSP (mGluR-EPSP). This mGluR-EPSP was blocked by the selective mGluR1 antagonists LY367385 and CPCCOEt, but not by the mGluR5 antagonist MPEP. Group II agonists affected neither iGluR-EPSP nor mGluR-EPSP. Conversely, L-AP4 and L-SOP, group III mGluR agonists, inhibited both iGluR- and mGluR-EPSPs. The depolarisations evoked by both AMPA and group I agonists were unaffected, indicating a presynaptic action of group III mGluRs. These data suggest that glutamate released by parallel fibres activates group III mGluR autoreceptors, depressing both iGluR- and mGluR1-mediated EPSPs. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
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