期刊
JOURNAL OF INFECTIOUS DISEASES
卷 184, 期 6, 页码 732-737出版社
OXFORD UNIV PRESS INC
DOI: 10.1086/322986
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资金
- NHLBI NIH HHS [HL-61951] Funding Source: Medline
- NIAID NIH HHS [AI-47225] Funding Source: Medline
- NINDS NIH HHS [NS-26310] Funding Source: Medline
Invasion of brain microvascular endothelial cells (BMECs) is a key step in the pathogenesis of meningitis due to Escherichia coli and Listeria monocytogenes. Although host cell actin cytoskeletal rearrangements are essential in BMEC invasion by E. coli K1 and L. monocytogenes, the underlying signaling mechanisms remain unclear. This study demonstrates that host cell cytosolic phospholipase A(2) (cPLA(2)) contributes to E. coli K1 invasion of BMECs but not to L. monocytogenes invasion of BMECs. This difference was observed with 4-bromophenacyl bromide, a nonselective PLA(2) inhibitor, and arachidonyl trifluoromethyl ketone, a selective cPLA(2) inhibitor, and was confirmed with BMEC derived from cPLA(2) knockout mice. Activation of cPLA(2) leads to generation of intracellular arachidonic acid, which is metabolized via cyclooxygenase (COX) and lipo-oxygenase (LOX) pathways into eicosanoids. COX and LOX inhibitors also significantly inhibit E. coli K1 invasion of BMECs.
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