期刊
JOURNAL OF CHROMATOGRAPHY A
卷 929, 期 1-2, 页码 31-42出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(01)01134-7
关键词
cytokinins; zeatin; dihydrozeatin isopentenyladenosine
Microcolumn liquid chromatography (mu LC) combined with electrospray tandem mass spectrometry is used for the determination of intact glycosylated cytokinins and the corresponding aglycons at picomole and sub-picomole levels in plant tissue. Routine analysis was done on C-8-bonded silica using a methanol-water gradient. Data acquisition was performed by multiple reaction monitoring, Quantification was carried out by using isotopically labelled analogues and applying linear regression to the response factor versus concentration data. For routine analysis a calibration range from 0.5 to 10 pmole injected on-column was used. The limits of detection ranged from 50 to 100 fmole injected on-column. The mu LC procedure was used to analyse plant tissue extracts from transgenic homozygote and hemizygote as well as wild-type Nicotiana tabacum species, and cauliflower samples. The data were compared with results obtained by conventional immunoassay and a satisfactory correlation was found. Validation data are presented. (C) 2001 Elsevier Science B.V. All rights reserved.
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