期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 98, 期 20, 页码 11193-11198出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.201407298
关键词
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资金
- NIGMS NIH HHS [R37 GM034277, R37-GM34277] Funding Source: Medline
Splicing of short introns by the nuclear pre-mRNA splicing machinery is thought to proceed via an intron definition mechanism, in which the 5 ' and 3 ' splice sites (5 ' ss, Tss, respectively) are initially recognized and paired across the intron. Here, we describe a computational analysis of sequence features involved in recognition of short introns by using available transcript data from five eukaryotes with complete or nearly complete genomic sequences. The information content of five different transcript features was measured by using methods from information theory, and Monte Carlo simulations were used to determine the amount of information required for accurate recognition of short introns in each organism. We conclude: (i) that short introns in Drosophila melanogaster and Caenorhabditis elegans contain essentially all of the information for their recognition by the splicing machinery, and computer programs that simulate splicing specificity can predict the exact boundaries of approximate to 95% of short introns in both organisms; (it) that in yeast, the 5 ' ss, branch signal, and 3 ' ss can accurately identify intron locations but do not precisely determine the location of 3 ' cleavage in every intron; and (iii) that the 5 ' ss, branch signal, and 3 ' ss are not sufficient to accurately identify short introns in plant and human transcripts, but that specific subsets of candidate intronic enhancer motifs can be identified in both human and Arabidopsis that contribute dramatically to the accuracy of splicing simulators.
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