期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 79, 期 1, 页码 33-38出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/S0925-4005(01)00845-0
关键词
biosensor; Prussian Blue; lactate oxidase; lactic acid; nation; flow-injection analysis
The properties of amperometric lactate biosensors based on Prussian Blue (PB) and lactate oxidases (LOX) of different origin (from Pediococcus sp. and Enterococcus sp. RL1) immobilised in Nafion were investigated by flow injection analysis. The operating potential was -50 mV (versus Ag/AgCl, 0.1 M KCl). A linear range in the dependence of current response upon the concentration of lactic acid was up to 0.8 mM. The detection limit of electrodes containing a high amount (6 U) of enzyme was below 1 muM. Operational stability could be maintained by exposing the electrodes to low (20 muM) concentrations of lactic acid. (C) 2001 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据