期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 256, 期 1-2, 页码 89-105出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(01)00433-1
关键词
rodent; T lymphocytes; cell trafficking; MRI, superparamagnetic iron oxide; homing; in vivo animal models
资金
- NCI NIH HHS [CA 20408] Funding Source: Medline
- NIAID NIH HHS [R01 AI46990, R01 AI42900] Funding Source: Medline
- NIAMS NIH HHS [N01-AR-6-2224] Funding Source: Medline
- NIA NIH HHS [R01 AG11653-6A2] Funding Source: Medline
The present study analyzed the feasibility of using magnetic resonance imaging (MRI) to monitor T-cell homing in vivo after loading T cells with superparamagnetic iron oxide (CLIO) nanoparticles derivatized with a peptide sequence from the transactivator protein (Tat) of HIV-1. T cells were isolated from C57BL/6 (B6) mice and loaded with 0, 400, 800, 1600, or 8000 ng/ml of FITC conjugated CLIO-Tat (FITC-CLIO-Tat). There was a dose-dependent uptake of FITC-CLIO-Tat by T cells. Stimulation of FITC-CLIO-Tat loaded T cells with anti-CD3 (0.1 mug/ml) plus IL-2 (5 ng/ml) elicited normal activation and activation-induced cell death (AICD) responses, and normal upregulation of CD69, ICAM-1 (CD54), L-selectin (CD62L), and Fas. The FITC-CLIO-Tat loaded T cells (3 X 10(7)) were transferred intravenously (i.v.) into B6 mice and the in vivo MRI of mice was acquired using a spin-echo pulse sequence at 4.7 T with a Bruker Biospec system. Homing of T cells into the spleen was observed by a decrease in MRI signal intensity within I It after the transfer, which remained decreased for 2-24 h after transfer. These homing data were confirmed by FACS analysis and biodistribution analysis using I-125-CLIO-Tat. Thus, T cells can be efficiently loaded with FITC-CLIO-Tat without interfering with their normal activation and AICD, or homing to the spleen, and the biodistribution of FITC-CLIO-Tat loaded T cells can be monitored in vivo over time by MRI. (C) 2001 Elsevier Science B.V. All rights reserved.
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