期刊
MICRON
卷 32, 期 7, 页码 691-700出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0968-4328(00)00066-4
关键词
nonlinear imaging; second-harmonic generation; two-photon; microscopy; numerical aperture; femtosecond pulses; resolution
类别
We describe the principles and characteristics of second-harmonic generation imaging (SHGI) and explore various methods for optimization of the technique. Second-harmonic imaging is optimized for ultrashort laser pulses, high numerical aperture microscope objectives, a highly sensitive non-descanned large area detector, pseudo-phase-matching, and specimens with large second-order non linearity or which exhibit surface plasmon enhanced phenomena. We also compare and contrast the techniques of SHGI and two-photon excited fluorescence imaging. (C) 2001 Elsevier Science Ltd. All rights reserved.
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