Chemical mechanism of β-xylosidase from Trichoderma reesei QM 9414:: pH-dependence of kinetic parameters

The variation of kinetic parameters of beta -xylosidase from Trichoderma reesei QM 9414 with pH was used to elucidate the chemical mechanism of the p-nitrophenyl beta -D-xylopyranoside hydrolysis. The pH-dependence of V and V/K-m showed that a group on the enzyme with a pK value of 3.20 must be unprotonated and a group with a pK value of 5.20 must be protonated for activity and both are involved in catalysis. Solvent-perturbation studies indicated that these groups are neutral acid type. Temperature dependence of kinetic parameters suggested the stickiness of the substrate at lower temperatures than the optimum and the calculated ionization enthalpies pointed to carboxyl groups as responsible for both pKs. Chemical modification with triethyloxonium tetrafluoroborate and protection with the substrate studies demonstrated essential carboxyl groups on the enzyme. Profiles of pf for D-gluconic acid lactone indicated that a group with a pK value of 3.45 must be protonated for binding and it has been assigned to the carboxyl group of D-gluconic acid formed by lactone ring breakdown in solution. (C) 2001 Societe francise de biochimie et biologie moleulaire/Editions scientifiques et medicales Elsevier SAS. All rights reserved.