期刊
PAIN
卷 94, 期 1, 页码 39-46出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0304-3959(01)00339-6
关键词
dorsal root ganglion; spontaneous activity; protein kinase A; neuropathic pain
资金
- NINDS NIH HHS [NS 12624, NS 10174] Funding Source: Medline
Protein kinase A (PKA) can play a critical role in the modulation of neuronal excitability. We examined the role of PKA in the modulation of abnormal spontaneous activity (SA) originating from the chronically compressed dorsal root gang ion (CCD). The L(4) and L(5) dorsal root ganglia (DRGs) were compressed by inserting a stainless steel rod into each corresponding intervertebral foramen. After 1-14 postoperative days, SA in DRG neurons with myelinated axons was recorded in vitro from teased dorsal root microfilaments. Rp-cAMPS (5-500 muM), a specific inhibitor of PKA, caused a dose-dependent decrease in the discharge rate of SA when topically applied to the DRG. The highest dose completely blocked the SA, but not the conduction of action potentials. H89 (10 muM), another PKA inhibitor, also markedly decreased SA. Sp-cAMPS (500 muM), a specific activator of PKA, increased the discharge rate of SA in all injured units tested, but did not trigger firing in silent neurons. Okadaic acid (0.1 muM), a protein phosphatase inhibitor, and forskolin (1 muM), an adenyl cyclase activator, each significantly increased the discharge rate of SA. These results strongly suggest that PKA modulates the SA in injured DRG neurons with myelinated axons. (C) 2001 International Association for the Study of Pain. Published by Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据