期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 12, 期 5, 页码 473-480出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0958-1669(00)00248-2
关键词
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Site-specific recombinases have become powerful tools for the targeted integration of transgenes into defined chromosomal loci. They have been successfully used both to achieve predictable gene expression in cell culture and for the systematic creation of transgenic animals. A recent improvement of this method, the recombinase-mediated cassette exchange procedure (RMCE), permits expression in the absence of any co-expressed selection marker gene.
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