期刊
EUROPEAN JOURNAL OF BIOCHEMISTRY
卷 268, 期 19, 页码 5142-5148出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1046/j.0014-2956.2001.02449.x
关键词
myeloperoxidase; eosinophil peroxidase; compound I; standard reduction potential; stopped-flow kinetics
The standard reduction potential of the redox couple compound I/native enzyme has been determined for human myeloperoxidase WO) and eosinophil peroxidase (EPO) at pH 7.0 and 25 degreesC. This was achieved by rapid mixing of peroxidases with either hydrogen peroxide or hypochlorous acid and measuring spectrophotometrically concentrations of the reacting species and products at equilibrium. By using hydrogen peroxide, the standard reduction potential at pH 7.0 and 25 degreesC was 1.16 +/- 0.01 V for MPO and 1.10 +/- 0.01 V for EPO, independently of the concentration of hydrogen peroxide and peroxidases. In the case of hypochlorous acid, standard reduction potentials were dependent on the hypochlorous acid concentration used. They ranged from 1.16 V at low hypochlorous acid to 1.09 V at higher hypochlorous acid for MPO and from 1.10 V to 1.03 V for EPO. Thus, consistent results for the standard reduction potentials of redox couple compound I/native enzyme of both peroxidases were obtained with all hydrogen peroxide and at low hypochlorous acid concentrations: possible reasons for the deviation at higher concentrations of hypochlorous acid are discussed. They include instability of hypochlorous acid, reactions of hypochlorous acid with different amino-acid side chains in peroxidases as well as the appearance of a compound I-chloride complex.
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