期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 49, 期 10, 页码 1277-1284出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/002215540104901010
关键词
mitochondrial membrane potential; apoptosis; granulosa cells; CM-H-2 TMRos; cytochrome c
类别
Dissipation of mitochondrial membrane potential (Delta Psim) and release of cytochrome c from mitochondria appear to be key events during apoptosis. The precise relationship (cause or consequence) between both is currently unclear. We previously showed in a model of serum-free cultured granulosa explants that cytochrome c is retained in a subset of respiring mitochondria until late in the apoptotic process. in this study we further investigated the issue of heterogeneity by using the Delta Psim-sensitive probe CM-H(2)TMRos in combination with a DNA fluorochrome. Changes of Delta Psim were assessed qualitatively by epifluorescence microscopy and were quantified using digital imaging microscopy. This approach yielded the following results: (a) CM-H2TMRos staining is a reliable and specific procedure to detect Delta Psim changes in granulosa cells explants; (b) dissipation of transmembrane potential is an early event during apoptosis preceding nuclear changes but is confined to a subpopulation of mitochondria within an individual cell; (c) in frankly apoptotic cells a few polarized mitochondria can be detected. These findings support the hypothesis that ATP needed for completion of the apoptotic cascade can be generated during apoptosis in a subset of respiring mitochondria and is not necessarily derived from anaerobic glycolysis.
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