期刊
MOLECULAR GENETICS AND GENOMICS
卷 266, 期 2, 页码 231-238出版社
SPRINGER-VERLAG
DOI: 10.1007/s004380100545
关键词
ATP citrate lyase; Cyanophora paradoxa; compartmentation; fatty acid biosynthesis; evolution
The single-copy gene for the large subunit of ATP citrate lyase (ACL) from the alga Cyanophora paradoxa was characterized at the cDNA and genome levels. The gene product showed high sequence similarity to its mammalian counterparts, but is smaller in size, as is also found for the fungal subunit Acll and, most probably, for the corresponding subunit from Arabidopsis thaliana. The C. paradoxa gene is interrupted by at least 12 introns of 53-65 by with conserved border and branchpoint sequences, and the product lacks a stroma-targeting peptide. Enzyme activity was found in the cytosol of C. paradoxa but not in the plastid (cyanelle) fraction. This is in contrast to the subcellular distribution of ATP citrate lyases in higher plants, where both chloroplast and cytosolic enzymes have been reported.
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