期刊
ARCHIVES OF MICROBIOLOGY
卷 176, 期 4, 页码 306-309出版社
SPRINGER-VERLAG
DOI: 10.1007/s002030100329
关键词
sulfate-reducing bacteria; aerobic respiration; terminal oxidase; cytochrome c(3); periplasm; hydrogen; TMPD; ascorbate; Desulfovibrio vulgaris strain Marburg Desulfovibrio desulfuricans strain CSN Desulfovibrio termitidis strain KH1
类别
Washed cells of Desulfovibrio vulgaris strain Marburg (DSM 2119) reduced oxygen to water with H, as electron donor at a mean rate of 253 nmol O-2 min(-1) (mg protein)(-1). After separating the periplasm from the cells, more than 60% of the cytochrome c activity and 90% of the oxygen-reducing activity were found in the periplasmic fraction. Oxygen reduction and the reduction of cytochrome c with H, were inhibited by CuCl2. After further separation of the periplasm by ultrafiltration (exclusion sizes 30. 50, and 100 kDa), oxygen reduction with H-2 occurred with the retentates only. Ascorbate plus tetramethyl-p-phenylenediamine (TMPD), however, were also oxidized by the filtrates. The stoichiometry of 1 mol O-2 reduced per 2 mol ascorbate oxidized indicated the formation of water. Our experiments present evidence that in D. vulgaris periplasmic hydrogenase and cytochrome c play a major role in oxygen reduction. Preliminary studies with other Desulfovibrio species indicated a similar function of periplasmic c-type cytochromes in D. desulfuricans CSN and D. termitidis KH1.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据