期刊
EXPERIMENTAL PARASITOLOGY
卷 99, 期 2, 页码 66-72出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/expr.2001.4657
关键词
Boophilus microplus; Ixodidae; catalase; colony forming units; ethylenediaminetetraacetic acid; hydrogen peroxide; lipopolysaccharide; reduced nicotinamide adenine dinucleotide phosphates; nitric oxide; phosphate-buffered saline; supplemented phosphate-buffered saline; phorbol 12-miristate 13-acetate; peroxidase; reactive oxygen species; superoxide dismutase
类别
We have investigated the phagocytic activity and the production of reactive oxygen species (ROS) by hemocytes from the cattle tick Boophilus microplus. Two main types of hemocytes were detected in tick hemolymph: plasmatocytes and granulocytes. The plasmocytes were the most abundant cells. being responsible for the in vivo phagocytosis of yeast. ROS production was evaluated by luminol-amplified luminescence and phenol red oxidation. The luminescence increased when hemocytes were incubated with bacteria, zymosan, or phorbol 12-miristate 13-acetate (PMA). The luminescence was inhibited by superoxide dismutase and catalase. which are antioxidant enzymes that remove superoxide and hydrogen peroxide, respectively. The phenol red oxidation assay also showed an increase in the level of hydrogen peroxide produced by hemocytes stimulated with bacteria and PMA. Taken all together. our data indicate that tick hemocytes are able to produce ROS during the phagocytic process similarly to vertebrate phagocytes. (C) 2001 Elsevier Science.
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