Antioxidative activity of 3,4-dihydroxyphenylacetic acid and caffeic acid in rat plasma

The purpose of the present paper is to study and compare in vitro the inhibitory effect of 3,4-dihydroxyphenyl-acetic acid (DOPAC) and caffeic acid (CA) on lipid peroxidation in rat plasma, Rat plasma was oxidized at 37 degreesC by the radical initiators 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) or 2,2'-azobis(4-methoxy-2,4-dimethylvaleronitrile) (MeO-AMVN). The consumption of endogenous alpha -tocopherol (alpha -TOH) and the accumulation of conjugated diene hydroperoxides were measured by high-performance liquid chromatography and by ultraviolet spectroscopy, respectively. alpha -TOH was consumed at the same rate in the presence of 20 mM AAPH or 2 mM MeO-AMVN. DOPAC and CA suppressed the alpha -TOH consumption in a dose-dependent manner. A concentration of 50 muM of both phenolic acids was sufficient to induce a lag phase and to delay the rate of alpha -TOH consumption. The effect was more pronounced in rat plasma oxidation by AAPH than by MeO-AMVN. CA spared vitamin E more effectively than DOPAC in both oxidations. DOPAC and CA suppressed the formation of conjugated diene hydroperoxides. DOPAC and CA at concentration 50 muM suppressed alpha -TOH consumption during oxidation of soybean phosphatidylcholine (2.8 mM) multilamellar vesicles containing 15 muM alpha -TOH, in which the lipophilic initiator 2,2'-azobis (2,4-dimethylvaleronitrile) (6 mM) was incorporated. In conclusion, we demonstrated that DOPAC and CA in micromolar concentrations have antioxidant activity in rat plasma, a medium very close to the conditions in vivo, suggesting that supplementation with the phenolic acids will provide significant antioxidant protection.