期刊
NATURE MEDICINE
卷 7, 期 10, 页码 1159-1162出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nm1001-1159
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Successful adoptive T-cell therapy has been demonstrated in viral disease(1,2) and selected forms of cancer(3). However, it is limited by the difficulty to efficiently isolate and amplify autologous tumor-reactive T-cell clones. Tetramers of major histocompatibility complex (MHC) class I and peptide have facilitated the characterization of CD8(+) T cells specific for tumor-associated antigens(4,5). However, for adoptive T-cell therapy, MHC-tetramers have limitations: they require knowledge of tumor antigens, which is often not available; they select T cells with a single specificity, thereby posing risk for selection of tumor escape variants; they do not select for function, so that T cells may be anergic when isolated from cancer patients; and they do not allow the isolation of CD4(+) T cells that can be essential for tumor rejection(6). Because interferon (IFN)-gamma is essential for tumor rejection(7,8), we isolated live T cells based on their IFN-gamma production(9). IFN-gamma secreted by previously activated T cells is retained on the cell surface, allowing their specific isolation and expansion(10). We show here that IFN-gamma (+) but not IFN-gamma (-) T cells from tumor-immunized mice are cytolytic and mediate tumor rejection upon adoptive transfer. Importantly, tumor-specific T cells can be enriched from lymphocytes infiltrating human renal cell carcinoma by the IFN-gamma capture assay.
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