期刊
JOURNAL OF ECONOMIC ENTOMOLOGY
卷 94, 期 5, 页码 1177-1182出版社
OXFORD UNIV PRESS INC
DOI: 10.1603/0022-0493-94.5.1177
关键词
Agromyzidae; polymerase chain reaction-restriction fragment-length polymorphism; molecular identification; pea leafminer; introduced species; invasive species
类别
A molecular method is presented for differentiating the morphologically cryptic leafminers Liriomyza langei Frick and L. huidobrensis (Blanchard). This method requires polymerase chain reaction (PCR) amplification of a 1031-bp region of mitochondrial cytochrome oxidase DNA followed by restriction fragment analysis using the restriction enzymes SpeI and EcoRV. SpeI cuts the mitochondrial fragment of L. langei into two fragments, but does not cut the L. huidobrensis fragment. EcoRV cuts the L. huidobrensis fragment into two fragments, but does not cut the L. langei fragment. This PCR-restriction fragment-length polymorphism (RFLP) method is faster and less costly than DNA sequencing, which is currently the only other way to differentiate these two species. We apply the method to samples from recently introduced leafminer populations in Sri Lanka, Canada, and South Africa and find that the invasive leafminer in all three locations is L. huidobrensis.
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