期刊
JOURNAL OF NEUROSCIENCE
卷 21, 期 19, 页码 7576-7586出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.21-19-07576.2001
关键词
familial amyloidotic polyneuropathy; amyloid; transthyretin; RAGE; caspase-3; inducible nitric oxide synthase; inflammatory cytokine
资金
- NIA NIH HHS [AG16223, AG17490] Funding Source: Medline
Familial amyloid polyneuropathy (FAP) is a neurodegenerative disorder associated with extracellular deposition of mutant transthyretin (TTR) amyloid fibrils, particularly in the peripheral nervous system. We have hypothesized that binding of TTR fibrils to the receptor for advanced glycation end products (RAGE) on critical cellular targets is associated with a destructive stress response underlying peripheral nerve dysfunction. Analysis of nerve biopsy samples from patients with FAP (n = 16) at different stages of disease (0-3), compared with age-matched controls (n = 4), by semiquantitative immunohistology and in situ hybridization showed increased levels of RAGE, beginning at the earliest stages of the disease (FAP 0; p < 0.02) and especially localized In axons. Upregulation of proinflammatory cytokines (tumor necrosis factor-a and interleukin-1) (approximately threefold; p < 0.02) and the inducible form of nitric oxide synthase (iNOS) (2.5-fold; p < 0.04) was also observed in a distribution overlapping RAGE expression. Tyrosine nitration and increased activated caspase-3 in axons from FAP patients (p < 0.03) were apparent. Although these data suggest the presence of ongoing neuronal stress, there was no upregulation of neurotrophins (nerve growth factor and neurotrophin-3) in FAP nerves. Studies on cultured neuronal-like, Schwann, and endothelial cells incubated with TTR fibrils displayed RAGE-dependent expression of cytokines and iNOS at early times (6 and 12 hr, respectively), followed by later (24 hr) activation of caspase-3 and DNA fragmentation. We propose that the interaction of TTR fibrils with RAGE may contribute to cellular stress and toxicity in FAR Furthermore, there is an apparent lack of responsiveness of Schwann cells in FAP nerve to provide neurotrophic factors.
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