期刊
RHEUMATOLOGY
卷 40, 期 10, 页码 1163-1168出版社
OXFORD UNIV PRESS
DOI: 10.1093/rheumatology/40.10.1163
关键词
BMP; ossification; spinal ligament; RT-PCR; in situ hybridization; alkaline phosphatase
类别
Objective. To investigate the function of bone morphogenic protein-2 (BMP-2) in the ossification of the spinal ligament (OSL). Methods. Total RNA was prepared from the cultured spinal ligament cells from patients with OSL and analysed by reverse transcription-polymerase chain reaction using specific primers for BMP-2. BMP-2 mRNA expression in ligament tissues was examined by in situ hybridization. Spinal ligament cells from patients without OSL were treated with BMP-2 and examined for alkaline phosphatase activity. Results. Expression of the BMP-2 gene was detected in cultured spinal ligament cells. In ligament tissues, BMP-2 mRNA was present in the chondrocyte-like cells in the fibrocartilage zone. Exogenous BMP-2 increased alkaline phosphatase activity in spinal ligament cells from patients without OSL. Conclusion. The BMP-2 gene is expressed in the spinal ligaments of OSL patients, and exogenous BMP-2 stimulates osteogenic differentiation of spinal ligament cells. The expression of BMP-2 in the spinal ligaments could be a clue in elucidating how heterotrophic osteogenesis develops in ligament tissue.
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