期刊
BASIC RESEARCH IN CARDIOLOGY
卷 105, 期 3, 页码 365-377出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00395-010-0085-4
关键词
Lamin A/C; Thymopoietin; Mutation; Dilated cardiomyopathy; Cardiomyocyte; Nucleus ultrastructure
资金
- Canadian Institutes for Health Research [38054, 65152, 77685]
- Heart and Stroke Foundation [NA 5101, 6628, 5275]
- Institute of Cardiology (Warsaw, Poland) [2.57/VII/03]
- Astra Zeneca/Canadian Society of Hypertension/CIHR
- Ontario Graduate Scholarship in Science and Technology (OGSST)
Major nuclear envelope abnormalities, such as disruption and/or presence of intranuclear organelles, have rarely been described in cardiomyocytes from dilated cardiomyopathy (DCM) patients. In this study, we screened a series of 25 unrelated DCM patient samples for (a) cardiomyocyte nuclear abnormalities and (b) mutations in LMNA and TMPO as they are two DCM-causing genes that encode proteins involved in maintaining nuclear envelope architecture. Among the 25 heart samples investigated, we identified major cardiomyocyte nuclear abnormalities in 8 patients. Direct sequencing allowed the detection of three heterozygous LMNA mutations (p.D192G, p.Q353K and p.R541S) in three patients. By multiplex ligation-dependant probe amplification (MLPA)/quantitative real-time PCR, we found a heterozygous deletion encompassing exons 3-12 of the LMNA gene in one patient. Immunostaining demonstrated that this deletion led to a decrease in lamin A/C expression in cardiomyocytes from this patient. This LMNA deletion as well as the p.D192G mutation was found in patients displaying major cardiomyocyte nuclear envelope abnormalities, while the p.Q353K and p.R541S mutations were found in patients without specific nuclear envelope abnormalities. None of the DCM patients included in the study carried a mutation in the TMPO gene. Taken together, we found no evidence of a genotype-phenotype relationship between the onset and the severity of DCM, the presence of nuclear abnormalities and the presence or absence of LMNA mutations. We demonstrated that a large deletion in LMNA associated with reduced levels of the protein in the nuclear envelope suggesting a haploinsufficiency mechanism can lead to cardiomyocyte nuclear envelope disruption and thus underlie the pathogenesis of DCM.
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