期刊
CELLULAR IMMUNOLOGY
卷 213, 期 1, 页码 4-13出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/cimm.2001.1864
关键词
neuroblastoma; dendritic cells; cell fusion; cancer vaccine; cytotoxic T lymphocyte
The absence of surface costimulatory molecules explains in part the lack of an effective anti-tumor immune response in tumor-bearing animals, even though unique tumor antigens may be presented by class I MHC. We determined that the immunogenicity of a murine neuroblastoma, Neuro-2a, which lacks surface costimulatory molecules, could be increased by electrically induced fusion with dendritic cells. Electrofusion induced a higher level of cell fusion than polyethylene glycol, and tumor/dendritic cell heterokaryons expressed high levels of costimulatory molecules. While Neuro-2a was unable to induce the proliferation of syngeneic or allogeneic T cells in vitro, fused cells were able to induce T cell responses both in vitro and in vivo. When fused dendritic tumor cells were used as a cancer vaccine, immunized mice were significantly protected from challenge with Neuro-2a. We propose that electrofusion with patient-derived tumor and dendritic cells may provide a rapid means to produce patient-specific tumor vaccines. (C) 2001 Elsevier Science.
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