期刊
PHYSIOLOGICAL GENOMICS
卷 7, 期 1, 页码 55-63出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/physiolgenomics.2001.7.1.55
关键词
DNA microarray; endothelial cells; gene expression; shear stress
资金
- NHLBI NIH HHS [HL-56707, HL-60789, HL-64382] Funding Source: Medline
- NIEHS NIH HHS [ES-10337] Funding Source: Medline
The recently developed DNA microarray technology provides a powerful and efficient tool to rapidly compare the differential expression of a large number of genes. Using the DNA microarray approach, we investigated gene expression profiles in cultured human aortic endothelial cells (HAECs) in response to 24 h of laminar shear stress at 12 dyn/cm(2). This relatively long-term shearing of cultured HAECs led to the modulation of the expression of a number of genes. Several genes related to inflammation and EC proliferation were downregulated, suggesting that 24-h shearing may keep ECs in a relatively noninflammatory and nonproliferative state compared with static cells. Some genes were significantly upregulated by the 24-h shear stress; these includes genes involved in EC survival and angiogenesis (Tie2 and Flk-1) and vascular remodeling (matrix metalloproteinase 1). These results provide information on the profile of gene expression in shear-adapted ECs, which is the case for the native ECs in the straight part of the aorta in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据