期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 287, 期 5, 页码 1075-1082出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/bbrc.2001.5701
关键词
BAT3; Scythe; nuclear localization sequence; apoptosis; immunofluorescence; staurosporine; HeLa cells; caspases
Human Scythe (also known as BAT3) has been implicated in the control of apoptosis and regulating heat shock protein (HSP) 70 activity. We have attempted to further characterize the role of human Scythe in HeLa cells by studying the cellular localization and functional domains of a hemagglutinin (HA) epitope-tagged Scythe protein. Several HA-Scythe deletion mutant proteins were expressed in HeLa cells and their localization was detected using indirect immunofluorescence. Our data demonstrate that full-length human Scythe is a nuclear protein that contains an active C-terminal nuclear localization sequence (NLS). Site-directed mutagenesis of the NLS leads to complete nuclear exclusion of full-length Scythe. Furthermore, induction of apoptosis. by staurosporine does not cause redistribution or cleavage of Scythe, suggesting that Scythe remains localized in the nucleus during apoptosis. These results provide evidence that Scythe is a nuclear protein that probably does not interact with elements of the apoptotic machinery in the cytosol. (C) 2001 Academic Press.
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