期刊
JOURNAL OF CHROMATOGRAPHY A
卷 932, 期 1-2, 页码 55-64出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(01)01231-6
关键词
Trifolium pratense L.; red clover; stability; glucoside malonates; isoflavones
Isoflavones, their glucosides and their glucoside malonates were determined in red clover leaf extracts using reversed-phase LC coupled to atmospheric pressure chemical ionisation mass spectrometry (APCI-MS). UV and fluorescence detectors and the stability of the malonates was investigated. Extracts can be stored at least 1-2 weeks at -20 degreesC without loss of malonates. In LC-separated fractions the malonates are most stable when stored at low temperature after evaporation to dryness. The concentrations of eight major isoflavones ranged from 0.04 to 5 mg/g leaves. (C) 2001 Elsevier Science B.V. All rights reserved.
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