期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 394, 期 2, 页码 137-144出版社
ELSEVIER SCIENCE INC
DOI: 10.1006/abbi.2001.2514
关键词
Streptomyces peucetius var. caesius; glucose kinase; carbon catabolic repression
Glucose kinase catalyzes the ATP-dependent phosphorylation of glucose. Streptomyces peucetius var. caesius glucose kinase was purified 292-fold to homogeneity. The enzyme has cytosolic localization and is composed of four identical subunits, each of 31 kDa. The purified enzyme easily dissociates into dimers. However, in the presence of 100 mM glucose the enzyme maintains its tetrameric form. Maximum activity was found at 42 degreesC and pH 7.5. Isoelectric focusing of the enzyme showed a pl of 8.4. The N- and C-terminal amino acid sequences were MGLTIGVD and VY-FAREPDPIM, respectively. The kinetic mechanism of S. peucetius var. caesius glucose kinase appears to be a rapid equilibrium ordered type, i.e., ordered addition of substrates to the enzyme, where the first substrate is D-glucose. The K-m values for D-glucose and MgATP(2-) were 1.6 +/- 0.2 and 0.8 +/- 0.1 mM, respectively. Mg2+ in excess of 10 mM inhibits enzyme activity. (C) 2001 Academic Press.
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