期刊
JOURNAL OF CELL BIOLOGY
卷 155, 期 2, 页码 217-226出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200107058
关键词
peripherin; apoptosis; ALS; neuronal culture; TNF-alpha
类别
资金
- Wellcome Trust Funding Source: Medline
Peripherin, a neuronal intermediate filament protein associated with axonal spheroids in amyotrophic lateral sclerosis (ALS), induces the selective degeneration of motor neurons when overexpressed in transgenic mice. To further clarify the selectivity and mechanism of peripherin-induced neuronal death, we analyzed the effects of peripherin overexpression in primary neuronal cultures. Peripherin overexpression led to the formation of cytoplasmic protein aggregates and caused the death not only of motor neurons, but also of dorsal root ganglion (DRG) neurons that were cultured from dissociated spinal cords of peripherin transgenic embryos. Apoptosis of DRG neurons containing peripherin aggregates was dependent on the proinflammatory central nervous system environment of spinal cultures, rich in activated microglia, and required TNF-alpha. This synergistic proapoptotic effect may contribute to neuronal selectivity in ALS.
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