期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 276, 期 43, 页码 39788-39796出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M103227200
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The content and fine structure of keratan and chondroitin/dermatan sulfate in normal human corneas and corneas affected by macular corneal dystrophies (MCD) types I and II were examined by fluorophore-assisted carbohydrate electrophoresis. Normal tissues (n = 11) contained 15 mug of keratan sulfate and 8 mug of chondroitin/dermatan sulfate per mg dry weight. Keratan sulfates consisted of similar to4% unsulfated, 42%, monosulfated, and 54%, disulfated disaccharides with number of average chain lengths of similar to 14 disaccharides. Chondroitin/dermatan sulfates were significantly longer, similar to 40 disaccharides per chain, and consisted of similar to 64% unsulfated, 28% 4-sulfated, and 8% 6-sulfated disaccharides. The fine structural parameters were altered in all diseased tissues. Keratan sulfate chain size was reduced to 3-4 disaccharides; chain sulfation was absent in MCD type I corneas and cartilages, and sulfation of both GlcNAc and Gal was significantly reduced in MCD type IL Chondroitin/dermatan sulfate chain sizes were also decreased in all diseased corneas to similar to 15 disaccharides, and the contents of 4- and 6-sulfated disaccharides were proportionally increased. Tissue concentrations (nanomole of chains per mg dry weight) of all glycosaminoglycan types were affected in the disease types. Keratan sulfate chain concentrations were reduced by similar to 24 and similar to 75% in type I corneas and cartilages, respectively, and by similar to 50% in type II corneas. Conversely, chondroitin/dermatan sulfate chain concentrations were increased by 60-70% in types I and 11 corneas. Such changes imply a modified tissue content of individual proteoglycans and/or an altered efficiency of chain substitution on the core proteins, Together with the finding that hyaluronan, not normally present in healthy adult corneas, was also detected in both disease subtypes, the data support the conclusion that a wide range of keratocyte-specific proteoglycan and glycosaminoglycan remodeling processes are activated during degeneration of the stromal matrix in the macular corneal dystrophies.
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