期刊
FEBS LETTERS
卷 507, 期 2, 页码 187-190出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)02948-9
关键词
10-N-nonyl acridine orange; cardiolipin; fluorescence emission; cardiolipin pK; lipid phase separation; Escherichia coli
资金
- NIGMS NIH HHS [GM 54272] Funding Source: Medline
10-N-Nonyl acridine orange (NAO) has been used at low concentrations as a fluorescent indicator for cardiolipin (CL) in membranes and bilayers. The mechanism of its selective fluorescence in the presence of CL, and not any other phospholipids, is not understood. The dye might recognize CL by its high pK (pK(2) > 8.5). To investigate that, we established that NAO does not exhibit a pK in a pH range between 2.3 and 10.0. A second explanation is that the dye aggregates at hydrophobic domains on bilayers exposed by the CL. We found that a similar spectral shift occurs in the absence of CL in a concentrated solution of the dye in methanol and in the solid state. A model is proposed in which the nonyl group inserts in the bilayer at the hydrophobic surface generated by the presence of four chains on the phospholipid. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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