期刊
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
卷 49, 期 1-3, 页码 335-360出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-022X(01)00207-X
关键词
IMAC; immobilized-metal affinity chromatography; recombinant proteins; histidine tags; pharmaceutical proteins
Immobilized Metal-Affinity Chromatography (IMAC) represents a relatively new separation technique that is primarily appropriate for the purification of proteins with natural surface-exposed histidine residues and for recombinant proteins with engineered histidine tags or histidine clusters. Because the method has gained broad popularity in recent years, the main recent developments in the field of new sorbents, techniques and possible applications are discussed in this article. Advantages of the method and new prospects are described as well as the problems and concerns that appear when the method is to be used for production of pharmaceutical-grade proteins. (C) 2001 Elsevier Science B.V. All rights reserved.
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