The recovery of Arcobacter butzleri NCTC 12481 from various temperature treatments

Aims: The aim of this study was to determine the growth and survival characteristics for Arcobacter butzleri NCTC 12481. Methods and Results: The temperature and pH growth ranges were 15-39 degreesC and pH 6.0-8.0, as determined using impedance microbiology. The maximum specific growth rate was 00.57 h(-1) at 30 degreesC, pH 7.0. Arcobacter butzleri harvested from the exponential phase was more resistant to heat treatment than stationary phase cells (D-55 1.1 and 0.4 min, respectively). Fluorescent dye uptake, and the release of UV-absorbing material, increased in heat-treated cells. After 21 d storage at 4 and -20 degreesC, A. butzleri was recovered on blood agar, but not on the isolation media CAT or CCDA. Conclusion: Arcobacter butzleri cells from the exponential phase were less heat sensitive than those from the stationary phase. The organism was able to survive cold storage for at least 3 weeks. Significance and Impact of the Study: The growth and survival characteristics have been quantified thus providing a greater understanding of this newly emerging pathogen.