期刊
TRAFFIC
卷 2, 期 11, 页码 746-756出版社
MUNKSGAARD INT PUBL LTD
DOI: 10.1034/j.1600-0854.2001.21102.x
关键词
atomic force microscopy; AFM; cell mechanics; cytoskeleton; force measurements; imaging; membrane fusion; receptors; vesicles
类别
资金
- NIGMS NIH HHS [GM64020] Funding Source: Medline
Atomic force microscopy has emerged as a powerful tool for characterizing single biological macromolecules, macromolecular assemblies, and whole cells in aqueous buffer, in real time, and at molecular-scale spatial and force resolution. Many of the central elements of intracellular transport are tens to hundreds of nanometers in size and highly dynamic. Thus, atomic force microscopy provides a valuable means of addressing questions of structure and mechanism in intracellular transport. We begin this review of recent efforts to apply atomic force microscopy to problems in intracellular transport by discussing the technical principles behind atomic force microscopy. We then turn to three specific areas in which atomic force microscopy has been applied to problems with direct implications for intracellular trafficking: cytoskeletal structure and dynamics, vesicular transport, and receptor-ligand interactions. In each case, we discuss studies which use both intact cellular elements and reconstituted models. While many technical challenges remain, these studies point to several areas where atomic force microscopy can be used to provide valuable insight into intracellular transport at exquisite spatial and energetic resolution.
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